Reference Laboratories UKAS Accreditation

Reference Laboratories UKAS Accreditation

The only internationally recognised standard for testing laboratories that specifically demonstrates technical competence

Since 2004 our Wetherby Laboratory has held accreditation to BS EN ISO/IEC 17025:2000 (now updated to 17025:2005) general standards for the competence for testing and calibration laboratories granted by the United Kingdom Accreditation Service (UKAS).

UKAS Accreditation

The majority of tests in Biochemistry, Endocrinology, Haematology, Immunology, Microbiology, Histology and Anatomical pathology are included. In contrast to other quality standards such as ISO 9001:2000 and GLP (Good Laboratory Practice), BS EN ISO/IEC 17025 is the only internationally recognised standard for testing laboratories that specifically demonstrates technical competence, and the ability to generate technically valid results.

Technically valid results are ensured via the use of:

  • Validated testing methods, using suitable reagents and equipment which is regularly calibrated, checked and maintained to a high standard
  • Controlled Standard Operating Procedures (SOPs) for all test methods
  • Extensive quality control programmes including inter-laboratory comparisons
  • Appropriately qualified and properly trained staff, including technicians, veterinary pathologists, customer support and administration personnel
  • Full investigation into any problems that come to light, with appropriate corrective action taken to prevent reoccurrence and improve the way we work

Reference ranges provided with results for guidance are based on studies carried out in the laboratory wherever possible, but also take into account published information (which can reflect different methodologies and study populations). These are constantly under review by the Clinical Pathology team using experience from reporting cases and updated information from the veterinary field, and in our opinion offer the best aid in interpretation of reported results. as with all reference ranges some normal animals will lie outside these ranges.

The Quality Management System also requires that the business is managed in such a way as to ensure customer requirements are foremost. It is crucial that we understand our client expectations - we do this via customer surveys, courtesy calls and taking on board all comments received. If you would like to lodge a complaint or let us know how we can improve our service then please contact the Customer Support teams. We aim to resolve problems and, where appropriate, implement improved procedures as soon as possible. We will inform you of the outcome, including follow up contact, to ensure ongoing satisfaction, As a result, we put resources into those areas of our service that you consider to be of primary importance.

UKAS Accredited Tests/Methods

Revision no: 025 – Issue date 07 August 2015

For further reading on the UKAS accreditation please click here.

UKAS Accredited Tests

Sample Type Test Results Generated Equipment/Method
Whole blood Full blood count (including differential white blood cell count and blood film assessment)
Equine/Canine/Feline blood only
Red cells
Reticulocytes Haemoglobin Hct
White cells Neutrophils Lymphocytes Monocytes Eosinophils Basophils Platelets
SOP-92 (open tube) flow cytometry (colorimetric haemoglobin) using Advia 2120
WET-HAE-SOP-396 haemoglobin where necessary by colorimetric analysis using Hemocue Spectrophotometer
WET-HAE-SOP-395 film stain using Hema Tek slide stainer
WET-HAE-SOP-3667 Beckman slide maker stainer
Morphological assessment WET-HAE-SOP-399 and WET-HAE-SOP-401
manual film assessment/differential using light microscope
Buffy coat Preparation WET-HAE-SOP- 402 Buffy coat preparation where necessary, using Hawksley microhaematocrit centrifuge and slide stainer
Canine/Feline/Equine Coombs test Coombs test WET-HAE-SOP-411 using VMRD Canine Coombs kit
Haematocrit Haematocrit (Hct) WET-HAE-SOP-394 by centrifugation using Hawksley microhaematocrit centrifuge
Reticulocytes Reticulocytes WET-HAE-SOP-398 by Supravital staining and microscopy
Whole blood and serum Cross-matching Compatibility result WET-HAE-SOP-407 by haemagglutination and microscopy
Plasma (citrated) Fibrinogen Fibrinogen **WET-HAE-SOP-3336 by ACL ELITE using
optical coagulation
Prothrombin time Prothrombin time **WET-HAE-SOP-3336 by ACL ELITE using
optical coagulation
Partial thromboplastin time Partial thromboplastin time **WET-HAE-SOP-3336 by ACL ELITE using
optical coagulation
EDTA whole blood Canine and Feline Blood Grouping performance Canine and feline Blood Grouping WET-HAE-SOP-405 & 406 using Alvedia lab test Kit
Sample Type Test Results Generated Equipment/Method
Serum & Heparinised Plasma Bovine Viral Diarrhoea Virus (BVDV) Antibody
Bovine Viral Diarrhoea Virus (BVDV) Antigen
EDTA Plasma
Heartworm, Lyme and Ehrlichia by SNAP® 4Dx® Heartworm antigen Ehrlichia canis antibodies
Canine Lyme antibody
FIV antibody detection FIV antibody test WET-HAE-SOP-418 by IDEXX ELISA
FIV antibody detection FIV Western blot WET-HAE-SOP-419 by Western blot using IDEXX FIV kit
FeLV antigen detection Feline leukaemia virus WET-HAE-SOP-417 using IDEXX FeLV ELISA
Canine Pancreatic Lipase Canine Pancreatic Lipase Test WET-HAE-SOP-1928 using IDEXX CPLi kit
Canine Anti-Thyroglobulin Antibodies TGAA Test WET-HAE-SOP-2139 by ELISA
Rheumatoid Antibodies Canine
Antinuclear Antibodies
Rheumatoid Antibodies
Antinuclear Antibodies
WET-HAE-SOP-410 by latex agglutination
WET-HAE-SOP-409 by indirect immunofluorescence
Canine NT pro-BNP Canine pro-BNP WET-HAE-SOP-3463 by Canine Cardiopet Plus ELISA (IDEXX)
Feline NT pro-BNP Feline pro-BNP WET-HAE-SOP-3450 by Feline Cardiopet Plus ELISA (IDEXX)
Sample Type Test Results Generated Equipment/Method
Serum/Plasma Albumin Albumin Photometric, bromocresol green WET-BIO-SOP-509
Alkaline phosphatase Alkaline phosphatase 37C Photometric, IFCC EC
Alanine aminotransferase ALT (SGPT) 37C Photometric, IFCC without P5P
Aspartate aminotransferase AST(SGOT) 37C Photometric IFCC without
Amylase Amylase Photometric IFCC (EPS) EC
B-hydroxy-butyrate BHB Photometric, kinetic:
Bile acids Bile acids Photometric, L3K enzymatic recycling assay
Calcium Calcium Photometric, Arsenazo III
Cholesterol Cholesterol Photometric, CHOD-PAP
CK (CPK) CK (CPK) 37C Photometric, IFCC EC
Copper Copper Photometric
Creatinine Creatinine Photometric, Jaffe kinetic
Total bilirubin Total bilirubin Photometric, modified O'Leary
Fructosamine Fructosamine Photometric, NBT reduction
Gamma GT Gamma GT Photometric, IFCC EC
Glucose Glucose Photometric HK G6P-DH
Glutamate dehydrogenase (GLDH) GLDH DGCK EC
Inorganic phosphorus Inorganic phosphorus Photometric, molybdate, UV
Iron Iron Photometric, TPTZ
Lactate Dehydrogenase (LDH) LDH 37C Photometric, SCE EC
Lipase Lipase Photometric, enzymatic, colorimetric
Magnesium Magnesium Photometric, xylidyl blue
Total protein Total protein Photometric, blue violet
Sodium, potassium and chloride Sodium, potassium and chloride Indirect ISE
Triglycerides Triglycerides Photometric
Uric acid Uric acid Photometric, Uricase PAP
Urea Urea Photometric, Urease- GLDH
Urine Sodium, potassium and chloride Sodium, potassium and chloride Indirect ISE
Calcium Calcium Photometric, Arsenazo III
Creatinine Creatinine Photometric, Jaffe kinetic
Glucose Glucose Photometric HK G6P-DH
Inorganic phosphate Inorganic phosphate Photometric, molybdate, UV
Uric acid Uric acid Photometric, Uricase PAP
Urea Urea Photometric, Urease-GLDH
Urine/CSF Total Protein Total Protein Photometric, pyrogallol red/molybdate
    Helena SAS3 and 4
Serum Serum protein electrophoresis SPE WET-BIO-SOP-2676 by agarose gel electrophoresis
Sample Type Test Results Generated Equipment/Method
      Immulite 2000
Chemiluminescent competitive immunoassay
Serum/Plasma Cobalamin Cobalamin WET-BIO-SOP-499
Cortisol Cortisol WET-BIO-SOP-498
Folate Serum folate WET-BIO-SOP-500
Progesterone Progesterone WET-BIO-SOP-501
Thyroxine Thyroxine WET-BIO-SOP-497
Canine serum TSH Canine serum TSH WET-BIO-SOP-443
Digoxin Digoxin WET-BIO-SOP-448
Thyroxine Thyroxine Homogenouos enzyme immunoassay WET-BIO-SOP-2616 using Microgenics T4 assay
Phenobarbital Phenobarbital WET-BIO-SOP-2825
Sample Type Test Results Generated Equipment/Method
Animal swabs, tissues and biological fluids, as specified      
Swabs from eyes, nasal and throat, ears, skin, abscess/cyst, anal gland, interdigital cyst, pre- breeding, genitals Culture, isolation & antibiotic sensitivity of significant isolates Micro-organisms of veterinary clinical significance WET-MIC-SOP-2875
appropriate to the sample type with identification according to WET-MIC-SOP- 713.
Equine Genital Tract Samples Screening for Taylorella equigenitalis, Klebsiellapneumoniae & pseudomonas aeroginosa Presence of Taylorella equigenitalis, Klebsiella pneumoniae and Pseudomonas aeruginosa WET-MIC-SOP-674 as
screening for CEMO under the Code of Practice of the Horse Race Betting Levy Board
Blood cultures Fluids/Aspirates Culture, isolation & antibiotic sensitivity of significant isolates in blood cultures, fluids/ aspirates Micro-organisms of veterinary clinical significance WET-MIC-SOP-675
appropriate for the sample type
Aerobic and Microaerobic Organisms Isolated from Disease Situations Bacterial antibiotic sensitivity Susceptibility profile WET-MIC-SOP-2879 & 2880
using VITEK 2XL
Bacterial colonies from solid media Identification based upon the bacterial protein profile matching against a defined database Micro-organisms of veterinary clinical significance WET-MIC-SOP-3394 using the Bruker MALDI-TOF MS
Skin, hair and skin samples   Demonstrate presence of micro-organisms of veterinary clinical significance including bacteria, fungi, yeasts & ectoparasites WET-MIC-SOP-673 As
appropriate to the sample type
Urine Urine examination Urine chemistry, white blood cells, red blood cells, crystals, casts & epithelial cells WET-MIC-SOP-692 by
microscopy & dipsticks
Urine examination Micro-organisms of veterinary clinical significance WET-MIC-SOP- 692
Urine examination Specific Gravity WET-MIC-SOP-692 by
Faeces Faecal examination, culture & identification Salmonella spp WET-MIC-SOP-650 by
selective enrichment (Rappaports V broth) and selective plating (Brilliant Green Agar)
Faecal examination, culture & identification Campylobacter spp WET-MIC-SOP-650 by direct plating
Faecal examination Demonstration of parasites of veterinary clinical significance WET-MIC-SOP-751 using
direct microscopy, NaCl flotation and ZnSO4 flotation
Faecal examination Lungworm WET-MIC-SOP-751 by the
Baermann test
Faecal examination Worm egg count WET-MIC-SOP-751 using
direct microscopy
Sample Type Test Results Generated Equipment/Method
Fresh & fixed tissues and paraffin Wax blocks of processed tissue. Histology Interpretation of routine histological, histochemical and immunohistochemical stains including diagnosis for the purpose of disease identification. All below methods supported by documented procedures for cutting up, processing, embedding dehydration, clearing and mouting.
WET-HIS-SOP-205 & 49 Haematoxylin and Eosin (H&E) Stains
Identification of structures and cell morphologies through the detection of heparin granules, ferric iron, carbohydrates, basement membranes, connective tissue, copper, fungal elements and protozoa Special stains including; Gram Stain, Ziehl Neelson, Toluidine Blue, Perls Prussian Blue, Trichrome Stains, Melanin Bleach,  Congo Red, Rubeanic Acid, Periodic Acid Schiff,  Tissue Giemsa WET-HIS-SOP-206 – 211, 213, 215, 216, 236
Identification of specific cellular proteins including T and B cells melanocytes, cytokeratins, LI molecule and Corona Virus in macrohpages through antibody/antigen binding Immunohistochemistry WET-HIS-SOP-233 incorporating the following antibodies CD3, CD79, CK, VIM, Melan-A, Ki67, MAC 387, Lambda, FIP, CD117.
Opinions and Interpretations Interpretation of histology and immunohistochemistry staining including diagnosis for the purposes of disease identification in companion animals. Reporting  according to WET-ANP-SOP-1586
Sample Type Test Results Generated Equipment/Method
Aspirated Systemic Fluids Incl. (pleural/ascetic/pericardial/synovial)
Total Protein Total Protein AU2700
Refractive Index
WET-CYT-SOP-600 using a refractometer.

Referral Testing

Download a list of referred tests